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. 2014 Mar 20;9(3):e89963. doi: 10.1371/journal.pone.0089963

Table 1. EV-A71 primers and probe sequences.

oligo 5′-3′ sequences position
Tan et al. 2008 Forward primer GAGAGTTCTATAGGGGACAGT 2466–2489
Reverse primer AGCTGTGCTATGTGAATTAGGAA 2669–2647
probe 6FAM-ACTTACCCAGGCCCTGCCAGCTCC-TAMRA 2498–2521
Modified sequences Forward primer GARAGTTCYATAGGRGAYAGY 2466–2489
Reverse primer AGCTGTGCTRTGYGARTTRAGRA 2669–2647
Probe FAM-ATTGGRGCDTCRTCAAATGCTAGTGA-TAMRA 2595–2620

In the first three lines is the system developed by Tan et al. (2008b) and below the modified sequences that we used in this article for the detection of EV-A71.