Table 4. Results in Ct of the Taqman real-time PCR performed on plasmids A, B0 C1.

Probe	50 nM	100 nM	250 nM
Subgenogroup A	no	no	no
Subgenogroup B0	30.91	31.21	28.42
Gentype C1	32.19	31.75	31.84

Plasmids A, B0 and C1, at 10⁻⁵ dilution each, were tested with 2 µM of each primer at Tm 50°C and different concentrations of probe.