Table 6. Results in Ct of SYBR Green real-time PCR with Tm 44.6°C, 50.2°C and 55°C.
| Plasmids (C) | Tm 44.6°C | Tm 50.2°C | Tm 55°C |
| A (4×105) | 25.21 | 24.21 | 24.30 |
| B0 (8.5×105) | 26.21 | 23.86 | 23.80 |
| B1 (4.1×105) | 24.63 | 22.93 | 23.80 |
| B2 (6.5×105) | 23.25 | 22.45 | 25.93 |
| B3 (6×105) | 23.27 | 22.49 | 24.89 |
| B4 (4.4×105) | 24.87 | 24.88 | 29.33 |
| B5 (9×105) | 25.31 | 23.90 | 26.37 |
| C1 (4×106) | 26.36 | 24.36 | 25.93 |
| C2 (5.5×105) | 24.65 | 23.05 | 23.64 |
| C3 (6×105) | 25.01 | 22.89 | 25.14 |
| C4 (7×105) | 23.53 | 22.76 | 25.16 |
| C5 (9×105) | 26.28 | 23.40 | 24.33 |
All plasmids, at 10−5 dilution each, were tested with 2 µM of each primer. Cells filled in bold indicate lowest Ct value for each plasmid. ‘C’: number of plasmid copies used as template.