Figure 2.

Morroniside antagonizes H₂O₂-induced apoptosis in HELF cells but not in A549 cells. (A) Flow cytometry of HELF apoptosis. In 300 μM H₂O₂-treated HELF cells, the apoptotic rate was 28.3% following 24-h culture, while the addition of 100 μg/ml morroniside 2 h prior to H₂O₂ treatment inhibited HELF cell apoptosis. (B) Morroniside did not inhibit the apoptosis of HELF cells induced by staurosporine. Following 0.2 nM staurosporine treatment of HELF cells, the apoptotic rate was 8.1% after 24-h treatment. However, the addition of 100 μM morroniside 2 h prior to staurosporine treatment showed a similar apoptotic rate of 8.3%. M, morronside; S, staurosporine; HELF, human embryonic lung fibroblast. Blue, apoptotic cells; red, normal cells.