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. 2014 Mar 20;9(3):e92330. doi: 10.1371/journal.pone.0092330

Figure 3. Thermodynamic binding curve of SYP and RCP.

Figure 3

(A) Values of FRET efficiency in each group of samples were detected. The blue line presents the binding of RCP with increasing concentrations of SYP fitting a curve of single-site binding equation. The red line presents the binding of RCP with increasing concentrations of YFP as reference. Data represent the means ± SEM of three independent measurements. (B) Redox status of RCP and purity of the FRET-based probes were measured by non-reducing SDS-PAGE. Lines shows purified oxidized RCP (through exposed to air for 2 hours), reduced RCP (through dialysis in DTT containing binding buffer), purified SYP, the unstained protein molecular weight marker (Thermo Scientific).