Table 1. Sequences of PCR primers for construction of corresponding plasmids.
Sequence | Oligonucleotides (5'-3') | Restriction site |
rsrAA | CATGCCATGGGCAGCTGCGGAGAGCCG | NcoI |
rsrAB | CCGGAATTCGGACTCCTGCGGGGCCG | EcoRI |
sigRC | CATGCCATGGGCACTGGGACCGACGCA | NcoI |
sigRD | GGGAAGCTTTGACCCCGAGCCTTTCG | HindIII |
cfpA | CGGAATTCGGAGGAAGCATGGTGAGCAAGGGCG | EcoRI |
cfpB | CCCAAGCTTCTTGTACAGCTCGTCCATGC | HindIII |
yfpC | CCCAAGCTTGGAGGAAGCATGGTGAGCAAGGGCGA | HindIII |
yfpD | CCGCTCGAGCTTGTACAGCTCGTCCATGCC | XhoI |