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. 2014 Mar 20;10(3):e1004012. doi: 10.1371/journal.ppat.1004012

Figure 7. DHX36 facilitates PKR activation through its helicase/ATPase activity.

Figure 7

(A–B) HEK293T cells were transfected with empty vector, expression vector for HA-DHX36, or HA-DHX36 E335A. The cells were mock-treated or infected with NDV. After 12 h infection, cells were harvested and analyzed for phosphorylation of PKR by immunoblotting (A). Quantification of the signals for phospho-PKR is shown (B). (C–D) HeLa cells were transfected with control siRNA or that targeting human DHX36. Whole-cell lysates were prepared and pulled down with empty- (Beads) or poly I∶C-Beads (pIC-Beads). The precipitated proteins (Pull down) were analyzed by immunoblotting using the indicated antibodies. Protein expression was confirmed by immunoblotting using whole-cell lysate (WCL) (C). Quantification of the signals for RIG-I, PKR or phospho-PKR is shown (D).