Skip to main content
. 2014 Mar 20;9(3):e92451. doi: 10.1371/journal.pone.0092451

Figure 1. Assay set-up and autosampler capacity of two array-based biosensors.

Figure 1

(A) A CFM is used to print a microarray of 96 mAbs onto a sensor chip, which is then loaded into a SPRi reader. In the image of the 96-ligand array shown here, the red squares define the reaction spots (named by mAb) and the green rectangles define the interstitial reference spots (two per adjacent reaction spot). The SPRi is equipped with an autosampler that injects an analyte and cycles it back-and-forth across the flow cell. Up to 96 analytes are accommodated in a 96-well microplate and common reagents (i.e., antigen, buffer, and regeneration) are accommodated in 11-ml vials. (B) In the BLI system, 96 ligand-coated fiber optic sensors are dipped in parallel into a 96-analyte array. Analytes and common reagents are distributed across two 384-well microplates. The sample layout depicts that used for a typical classical sandwich epitope binning assay (see Table 1 ).