Figure 7. acs-3(ft5) mutants are mildly sensitive to hypo-osmotic stress.

(A) Animals of the indicated genotype were added to a 50 μl drop of dH₂0 and viability was assessed every five minutes. Error bars represent standard deviation from 3–5 independent biological replicates with ten animals assayed per experiment (two-tailed T-test for the 20 min timepoint, p<0.05: WT vs acs-3, WT vs acs-20, WT vs acs-3;nhr-25, nhr-25 vs acs-3;nhr-25, and acs-3 vs acs-20). Detailed statistical analyses, including all pair-wise comparisons are presented in Figure S10B.