Figure 2.

Twist2 confers resistance to cisplatin in ovarian cancer cells. (A) Validating the efficiency of pcDNA3.1(+)/Twist2 in OV2008 cells by quantitative polymerase chain reaction (qPCR). PcDNA3.1(+)/Twist2 significantly increased Twist2 at mRNA levels. (B) Validating the efficiency of si-Twist2 in C13K cells by qPCR. si-Twist2 significantly ablated endogenous Twist2 at the mRNA level. ^*P<0.05. (C) Validating the efficiency of pcDNA3.1(+)/Twist2 in OV2008 cells by western blotting. PcDNA3.1(+)/Twist2 significantly upregulated Twist2 at the protein level. (D) The efficiency of si-Twist2 in C13K cells was validated by western blotting. si-Twist2 significantly ablated endogenous Twist2 at the protein level. (E) IC₅₀ level of OV2008/Twist2 induced by cisplatin was markedly higher than those of the OV2008 and OV2008/Vector cells. (F) IC₅₀ level of C13K/si-Twist2 induced by cisplatin was markedly lower than those of C13K and C13K/si-NC cells.