Figure 3.

Downregulation of SET leads to changes of the indicated protein levels in colorectal adenocarcinoma cells using western blot analysis. (A) Protein expression levels of β-catenin, c-Myc and PP2A in the LS174T, LS174T^NC, LS174T^RNAi, SW480, SW480NC and SW480^RNAi cells. β-catenin and c-Myc were downregulated, whereas PP2A was upregulated in (B) LS174T^RNAi and (C) SW480^RNAi cells. β-actin was used as a loading control. ^*P<0.05 and ^**P<0.01 when compared with the blank control and negative control groups respectively. Data are presented as the means ± standard deviation of triplicate cultures. RNAi, RNA interference; PPA2, protein phosphatase 2.