Table 2.
PKR - RNA interaction parameters derived from sedimentation velocity analysis in the presence of 5 mM Mg2+.
| RNA | Kd (µM) | s(RP)a | RMSb |
|---|---|---|---|
| VAIc | 0.334 (0.278, 0.401) | 7.18 (7.07, 7.29) | 0.00691 |
| AS | 1.71 (1.54, 1.90) | 4.82 (4.76, 4.89) | 0.00703 |
| TS | 5.04 (4.18, 6.10) | 4.03 (3.94, 4.12) | 0.00887 |
| CD1 | 134 (97.0, 212) | 5.37 (fixed)d | 0.00672 |
| CD2 | 62.9 (53.7, 75.4) | 5.44 (fixed)d | 0.00561 |
Parameters were obtained by global nonlinear least square analysis of sedimentation velocity measurements. The values in parentheses represent the 95% joint confidence intervals obtained using the F-statistic.
a
Uncorrected sedimementation coefficient (Svedbergs).
b
Root mean square deviation of the fit in absorbance units
c
Data were previously reported in reference 29.
d
The sedimentation coefficients of the complexes could not be determined by global analysis and were fixed assuming a frictional ratio of f/f0 = 1.4.