. Author manuscript; available in PMC: 2015 Mar 20.

Published in final edited form as: J Mol Biol. 2014 Jan 4;426(6):1285–1295. doi: 10.1016/j.jmb.2013.12.019

Table 2.

PKR - RNA interaction parameters derived from sedimentation velocity analysis in the presence of 5 mM Mg²⁺.

RNA	K_d (µM)	s(RP)^a	RMS^b
VAI^c	0.334 (0.278, 0.401)	7.18 (7.07, 7.29)	0.00691
AS	1.71 (1.54, 1.90)	4.82 (4.76, 4.89)	0.00703
TS	5.04 (4.18, 6.10)	4.03 (3.94, 4.12)	0.00887
CD1	134 (97.0, 212)	5.37 (fixed)^d	0.00672
CD2	62.9 (53.7, 75.4)	5.44 (fixed)^d	0.00561

Parameters were obtained by global nonlinear least square analysis of sedimentation velocity measurements. The values in parentheses represent the 95% joint confidence intervals obtained using the F-statistic.

Uncorrected sedimementation coefficient (Svedbergs).

Root mean square deviation of the fit in absorbance units

Data were previously reported in reference 29.

The sedimentation coefficients of the complexes could not be determined by global analysis and were fixed assuming a frictional ratio of f/f₀ = 1.4.