Skip to main content
. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Invest Dermatol. 2013 Sep 20;134(4):1033–1043. doi: 10.1038/jid.2013.401

Figure 1. PKP-1 promotes desmosome formation.

Figure 1

(a–d) Immunofluorescence images and (e, f) quantification of cell-cell border fluorescence intensity from keratinocytes expressing empty vector (EV) or PKP-1.myc in low (0.1mM) or high calcium media (0.6mM) for 24 hours and immunostained for surface Dsg3, total desmoplakin (DP) and myc (PKP-1.myc). Scale bar: 20Hm. (e, f) Mean ± SEM (n= 50 borders per group); *** p < 0.001 compared with EV (Mann Whitney). (g,h,i) Sequential detergent extraction and western blot analysis of Triton X-100 soluble (sol) and insoluble (insol) proteins. Quantification represents the mean of three independent experiments. (j) Electron micrographs and (k) quantification of desmosome lengths (n= 25 desmosomes per group); *** p < 0.001 compared with EV (Mann Whitney). Scale bars 1Hm.