| Gene | Forward Primer | Reverse Primer | PCR Conditiona | ENZYMEb |
|---|---|---|---|---|
| 1700001K19Rik | TGAAGGCATCTGGAGAGAG | TCTTCTGAGTCGCCATCATA | TD65HSP+2.0mM | HpyCH4IV |
| 2610528E23Rik | AGGTGTATCACACGCTTCTC | CAGGTCACTGACAGCATTTA | TD55HSP+0.5XQ | BsmAI |
| 4632411B12Rik | GAAAGATCAGAACCATCACT | GACTTAGGAGAACAGCTTGA | TD55HSP | HpyCH4III |
| 4930538K18Rik | AAAACACAGGAAGCGTAGG | ATGGCAATAGGGACGATGAC | TD65HSP | HphI |
| 5430411K18Rik | GTGGCTGGAACAGGTGTAC | GTCGTTCTGCTCCATCTGA | TD55HSP | MnlI |
| 9130404D08Rik | CCTGATCTGGGTCTGAGT | ATTCCAAAGCTCTGAGAAGT | TD55HSP | HpaII |
| Abcf2 | CTGCTCAGTGACACCAAACT | AACCCAATCCGTGTAAGATC | TD65HS+0.5XQ | 2%AG |
| Abr | CAAGAGAGGTCAGGAATG | GGACTGGAAACGGTACTT | TD55HSP+0.5XQ | AvaII |
| Acads | GAGCTGGCTGCCTCTGAG | GCTGGTGCCCTCGTAGAT | TD65HSP+0.5XQ | PvuII |
| Acbd3 | TGAACTCTTGCTGGGAACCAGGAT | TGCTGAGCTTCATTCAGTTGGAGC | 60HS+1XQ | SspI |
| Adam3 | ACTGGACATCGTATTTATCT | GGGTCCTAATTATTTGTGTA | TD65HSP | Ssp1 |
| Adamts1 | AAGAAACCAAAGCATTACAT | AAAGATTCTGCCCAGACTCT | TD55HSP+0.5XQ | MboI |
| Adck5 | GGTATGAGGAGGTGATGTCT | ATCGGCTCATAATCAAACTC | TD65HS+0.5XQ | MspI |
| Adra1d | CAACTATTTCATCGTGAACCTGG | TACACGCGGCAGTACATGAC | 54HS | BtgI |
| Afp | CAAGAAGACATCCCAACTAT | GACGTGTTTTTGCAGTTCT | TD65HSP+2.0mM | MspI |
| Allc | TTACTTCTCAGGGAGCTATG | CGCTAATGACTCCAAACTC | TD55HSP | AluI |
| Angpt4 | GCGACATGGACAATGATAAC | AGTGTTGATGGACTGGATAG | TD65HSP+2.0mM | MwoI |
| Ankrd10 | CTCCCAAAGATTCTGAGTCT | TACAGTCTGTTTCAGGTGAG | TD55HSP | NlaIV |
| Ankrd5 | ACAGAAGAAAAGAAAGTACA | GCAACTGCTATGACTTAGAG | TD65HSP+2.0mM | HaeIII |
| Apip | CATGACAGCAGCCTTAGAG | AAAAAAAAGCCAGTGTACT | TD55HSP+4mM | StyI |
| Arvcf | GACAATGAGGTCCGTGAG | ATGGGTCAGCGTCTGTAAG | TD65HSP | AvaI |
| Atp13a3 | ATCTACCTTTTTGACAAC | ACAGACCGATGACACTTA | TD55HSP | EcoRI |
| Atp1a1 | TTGCCTCCATTGTGACTG | CACTCTCAGCCTGTTCATAG | TD65HSP+2mM | BsaJI |
| Aup1 | GGAGCGGCTCTTTGACTC | GGTGCTACAGGTGGTGAG | TD65HSP+0.5XQ | Eco0109I |
| BC068157 | GGAGAGAGAAAGGATTTA | AGTCACAGCCCTTCTCTA | TD55HSP+2.0mM | ScrFI |
| Bcl2 | CACCCCTTCATCCAAGAATG | TACCCTGTTCTCCCTTG | HS58+1XQ | RsaI,12%AC |
| Btbd12 | CTGATGAAGTGGTCGAAGTT | CCGCTCCAGATTCAAGT | TD65HS | MboI |
| C330027C09Rik | AGTTCTTTGACAGCGATACT | GATGGTGAAGGATCAGATTA | TD65HSP | HindIII |
| Cage1 | TGGGGATATCTCAAGTCT | AGAATAACAAGTACCGAAGA | TD55HSP+4.0mM | MseI |
| Canx | TGTGGAATGCAGATGGGTGCTAGA | TGTTTGCTTCAAGGAGAACCCTGC | 60HS | BfaI |
| Car1 | CCTGGTCTTGATCTTGAACT | TATTTTGGCTCTCTGACTCA | TD65HS+0.5XQ | SfcI |
| Ccdc132 | GACAGTTAAAAACAGAGAAC | GGGATCTCATATCAATAAGA | TD55HS+1XQ | Cell IEXTRa |
| Cd24a | ATGCAAATCAATTCCATAAC | GAGAGCAGAATTACGTTGA | TD55HSP+0.5XQ | TaqI |
| Cdk20 | GCCTTTGAGTTCATGCTGTC | CAGGCCAAAGTCAGCTATCT | TD65HSP+2.0mM | NlaIII |
| Chst1 | CCATCACAGGACCAGTTA | ACAGCCTTCCAGGAACATT | TD55HSP+4mM | MboI |
| Ckm | AACACCCACAACAAGTTCAAGCTG | GTCTGGATGACATCGTCYASAGTG | TD55HSP+2.0mM | BstUI |
| Clec14a | ACTGGAAGCAAATAAATAAC | ACATTGGAACAGGAAGATAC | TD55HS+1XQ | NlaIII |
| Clp1 | TCCGAGGCTGCTTCTATC | GGTAGCACAGTGGAAGACAT | TD55HSP | AvaII |
| Clptml1 | GAATGTGATGGTGGATGACT | TATGGGGAGGTAGTGTACTG | TD65HS+0.5XQ | PstI |
| Cnot2 | TTATGTGGTCAGAAAATACA | CACAAAAGACTCAACACAAT | TD55HSP | DdeI |
| Col3a1 | GGACCAGGAAGTGATGGGAA | ACTTTCTCCTTGACTTCCCT | 60HS | 12%AC |
| Col6a1 | ATTGACATCCTCTTCGTGCT | CATCTGGTTGTGGCTGTACT | TD65HSP+0.5XQ | BstEII |
| Col9a1 | ATCAGGATTGGCCAAGATGA | GGAATCCTGAAGTCTACATT | 53HS+1XQ | RsaI |
| Cpvl | GGGCCTTATGTTATCACAAG | TAAAGCATGGAAAGGGTAGT | TD55HSP | HpaII |
| Crem | TTGCAGAGACAGATGATTCT | GAATNCCAGGCNCATCAGAG | TD65HSP+2.0mM | RsaI |
| Csf1r | GGGACAGCACGAGAATATAG | GTAGCAGCAGTATTCAGTGA | TD55HSP+2.0mM | DdeI |
| Csf3r | CTTTGCCACCAACATCTGG | GCTGGAAGGCTGATGTGAAG | TD55HSP | AvaII |
| Ddx23 | GCCGAACAAGACATCTCAAC | GTTCGCCTCTTTTCCATTAG | TD55HSP | MwoI |
| Dennd5a | TGTGAAGCACTTCCATAAAC | CACTCTCCACAAAGCAACAG | TD55HSP+2.0mM | AvaI |
| Dgcr14 | GTCGGGAGGGGATGTAGA | GAGGANAGCTGGCGGAAC | TD55HSP+0.5XQ | HinPI |
| Dnmt3a | TACAACAAGCAGCCCATGTACC | TCTCCTCTGCACCAAGAAGG | 59HS | BstU I |
| Dnttip2 | AAANTCAGGTGTAATGACAG | CATAGACCCTGGACTAAGTA | TD55HSP | AvaII |
| Dpm1 | TACCGAAAAGAAGTTCTAC | TGAAATGAACACGGTAAGT | TD55HSP | EarI |
| Dzip1 | GGGGATAAACCAGTTACT | TCCCGAGGGTTCAC | TD55HSP | 2%AG |
| Ecd | GGTGATGAAAGAGCACTAGAC | TGGTATCAGAAACAGGAAGTA | TD65HSP+1XQ | DraI |
| Ednrb | ATGACGCCACCCACTAAGAC | GATGATGCCTAGCACGAACA | 62; 2.0mM | Msp I |
| Efcab10 | AGTTATTCTATGTCCATCAT | AAAAAACCGAGAGAGTAT | TD55HSP | AvaII |
| Efhb | GCACTCAAAAGGTTCTGTTA | TGGAGCTTGAAATGAGACTA | TD55HSP | RsaI |
| Efr3a | ACAGGAAAAGGAAAAGAGAC | CCAATATTTGAGCAAGTCTA | TD55HS+1XQ | MnlI |
| Ell2 | AATCCGCCTCAGACTGTA | AGCGCAACCTTCTTTAACTC | TD65HSP+2.0mM | BglII;HindIII |
| Elmod2 | GTGAACTGGACAGATGTGTA | CCTGGTAGAGCTGCTTATAG | TD65HSP | RsaI |
| Esx1 | GTTTGGTTTCAGAACAGAAGG | GATATTTCCAAATAACCTCCACA | 52HS | MboI |
| Fbxw5 | CGGGGATGAGTTCTTAT | TGTAGCTCCAGTTGTATG | TD55HSP+0.5XQ | RsaI |
| Flt1 | CACAAAGATCCCAAAGAGA | AATGAAATGCTGGAGCTGTA | TD60HSP | EarI |
| Fn1 | AGAAGAGAGAGCCCCTGATTG | TGAAGATTGGGGTGTGGAAG | 54HSP | NcoI |
| Frzb | CTATGAAGAyGAGGAACGTTCCAG | CACTTCTCAGCTATAGAGCCTTC | 52; 2.0mM | MnlI |
| Fyn | TCTTTTTAGTTGAATCAGGT | CAGGTGGAGAGAGGTTACAG | TD55HSP | MnlI |
| Gabarapl1 | GAGCTGTAGGGGACCATAC | GGACGCTCTTCATTTGTAGA | TD55HSP | EarI |
| Gart | CAAGTAATAGCTAAAGGACA | GCTTTAGGACTCCAGGTATT | TD55HSP | HpyCH4V |
| Gast | CGCCTGCTCTGAAGCTTCTTG | GGCCGAAGTCCATCCATCC | 58HS | MseI,BsmAI |
| Gata6 | ATGGGTGTAAGAAGCATGTC | GTGGCAGAAACTGTGACAAT | TD55HSP+0.5XQ | TaqaI |
| Glra2 | TCAGGAAAATGCTGCCTCGT | ACACACCATCTCCATAAATACTGTAG | 59 | HpyCH4IV |
| Glul | AAGCAAGCGGCACCAGTACC | CACGTGCGGATGAGAGCTTCT | TD55HSP | NheI |
| Golim4 | TCCTGGTACTGTTCATCAAC | ATATAAACCCAGCAGATGAC | TD65HS+0.5XQ | HhaI |
| Gpi1 | GTGTGGACCACCAGACAGG | TTCCGTATGGGGTGCTGGGTC | TD55HSP+2.0mM | AciI |
| Gpx4 | GAATTCTCAGCCAAGGACAT | TTTTCATCCATTTCCACAGT | TD55HSP+0.5XQ | BsaJI |
| Gpx5 | CTGGAAATTAGGTACGTATC | AGAGCCAGGAGAAAATATAG | TD55HSP | StuI |
| Gramd3 | GGACATTTCTGAGACGTGAC | AAACCACCTCGGACAGA | TD55HSP+2.0mM | HpyCH4V |
| Grb10 | CATGCCAAATGAGAGTAA | GCAGGCACACATACAG | TD55HSP+0.5xQ | 2%AG |
| Grk4 | AGACCTAAAGCCAGAGAATA | GCTAGACCAAGATCTGAAAT | TD55HSP+2.0mM | SspI |
| H19 | GCACTAAGTCGTTTGCACTGG | CTGTTTCCAGACTAGGCGAG | 52 | AccI |
| Hba | CCCACCACCAAGACCTACTT | CGGTATTTGGAGGTCAGCAC | TD65HS | MspI |
| Heatr1 | GGGATTGATGGTCAGATCTA | CATCTAATGTTCTGGGGTAT | TD55HSP+1XQ | MseI |
| Hmox2 | GAAACATTAAGAAGGAGCTA | CTCATTCTGCCCTACATAGT | TD55HS | HinPI |
| Hps4 | ACCCCGATTTTCCTGAGT | CAGGGAGGGTTCTGAGAT | TD60HSP+0.5XQ | MboII |
| Hsbp1 | GTGACGGTTCCTAGACAT | GGACATGATCTGAAACTT | TD65HSP+0.5XQ | BfaI |
| Hsf2bp | GTATGCCTTCATGCCTTG | CCTGGTACTCAAGCACAC | TD65HSP | HaeIII |
| Hsp90b1 | GAATGACATCAAACCAATAT | TCCATATTCATCAAACAGAC | TD55HSP+2.5mM | EcoRI |
| Igfbp1 | TTTTACCTGCCAAACTGCAAC | GGTAGACGCACCAGCAGAG | TD55HSP | PvuII |
| Il23a | AGCCAGATCTGAGAAGCAGG | CTGCTCCRTGGGCAAAGACC | HS66 | MspI |
| Impact (#1) | TGGGTGAAAATATGGGCATT | AGGGCATGGACCACAGATAG | 49; 2.0mM | BfaI |
| Impact (#2) | CTGGTATGGAGGGATTCTGC | GCCCATATTTTCACCCAGTC | 49; 2.0mM | BssSI |
| Inha | TCAGCTCAGCTGTGGTTCC | CTCGTACTTGAAAGAGTAACCTCCA | TD55HSP | BssHII |
| Inpp4a | CAGCAGCTTCACAAACTG | CGTTGACGAGAGCTTGAC | TD65HSP+0.5XQ | DdeI |
| Irs4 | TAGGCTTGCTCCCGCTGGA | RAGCCCCGGCCATTTCCTGAGC | 64HS | Bme1580I |
| Isg20l2 | TTTCATCCCAAGTCTCTCAC | GCTAATTTTCTGGAGGATTC | TD65HSP | PstI |
| Itm2b | AGAAACCTGTTGGAGCTACT | GCTCATGGAGGAGGTAAG | TD65HSP+2.0mM | BamHI |
| Iws1 | TTGACAGTGGCGTGAT | ATTAATTTCCCTGCCATATC | TD55HSP | MboI |
| Katnb1 | AGGATGCCATGTCACAGATA | TTGCAGGAGTTTCTCAATCT | TD55HSP+0.5XQ | MspI |
| Lit1 | ATGTTGGAGGGAGGGGTATC | CCTTTCACAGGGGTCACCTA | TD55HSP+0.5xQ | HpyCH4III |
| Krt17 | GGAAGCCGACATCAATGG | TTCTTCAGGTAGGCCAGCTC | 52HS | MboI |
| Lama1 | GAGGGGTTTGCATCA | CCACAGGGTCACAGTTAC | HS59-64 | 2%AG |
| Lep | GACCTTAGCCCTGAATGCTG | TGCTTTGCTTCATATCCATCC | 52 | BsrBI |
| Lrch4 | ACCGAAGTCCAGTGCTAC | ACAACGGACTCTGGAGAG | TD65HSP | BccI |
| Ltbp1 | TAACACAGAGGGCTCTTACA | CCAGAGGACGGCTACA | TD55HSP | 2%AG |
| M33 | GCTCCCGTGTCATTTCTTTCAC | AGACAAGAGCAGTCATTCTGTCACC | 52 | length |
| Maoa | ATCAAGTGCATGGTGTATTACA | ATCTTCAATCATCATGCA | 52 | BstNI |
| Mapkapk5 | AGGAAACTGGACACTGTTAG | AAGAACGGTTTGCACTGA | TD55HSP | BsrI |
| Mas1 | GTGCACTGGGTCATYATGAGCAT | GCATTTCATCTTTGAAAGCCCTGGT | 62HSP | AvaII |
| Mdm1 | AAGTCCAAGGCAGACAGA | TTGTCTCAGCTTCGGAGTAG | TD65HSP | HinfI |
| Mlst8 | GGGCGGTAGCAGATGTAG | AGGCCCCACTGACAGTAAT | TD65HS | HphI |
| Mmel1 | CTTCAGAACATGGACAAATC | CGGTCTTTGACATCGGAATC | TD65HS+0.5XQ | HindIII |
| Mpo | CCACACCCTCATCCAACC | GTCCCCGGATCTCATCC | TD55HSP+2.0mM | PvuII |
| Mtus1 | GTAGATTCTCAAGCGACTTC | GCCCATGAAACCACTAA | TD65HSP | HaeIII |
| Mtx2 | TCTCTGAGAAAGGGCTTGA | GATGGGGGAACAAGACTCT | TD55HSP | BglII |
| Mut | TACTGTGGAAGAAAGCAATA | AGGGTTGTCTGAATCATAAC | TD55HS+1XQ | RsaI |
| Myl4 | AAGGAGCAGGGCACCTATG | GATGCAGCCATTGGCATC | 60HS | NheI |
| Nap1l5 | GCAGGATCTCTCTGGACCAGCTC | CCGTGTAGCTCCTGGATCTTGGC | 62 | BtsI |
| Nck1 | AGTATCACTAAAAGCACAAG | TNTCATACAGTACCAAACAG | TD55HSP/4.0mM | MboI |
| Oprk1 | CCTGGCTTTGGCRGATGCTTTRGT | TTCCCTGACTTTGGTGCCTCCAAG | 60HS | RsaI |
| Otc | ATGCTATGGCTATCAGCAGATCTGAAA | AGACAATCTTGTTCGAGTACTTCT | 55 | Fnu4HI |
| Pacrg | GAGGAAGAGCTGTACAAGTC | GACTACAGCCAGCAGAAGAG | TD65HSP+0.5XQ | Fnu4HI |
| Pacs2 | CACAATTCCAACCTTCACA | GGTTCATTCCCTTTGTTG | TD55HSP | AvaII |
| Pam | CATTCAGATTCCGAAAAATAGT | GAATCGGAGGAGGAGGAGTACT | TD55HSP+0.5XQ | HinFI |
| Pcnx | AGATTCTGGAAGGGATTAAC | TGACAGTCACGTATTTATCA | TD55HS+1XQ | MboI |
| Pdcl2 | TAAGAGATTTTGGCATTC | TCATCTTCTCATACGGTTTA | TD55HS+2.0mM | NlaIII |
| Pde9a | CTTCCAGTACCTGCTCTC | GGCTCTGTCTCTTGTGTC | TD65HSP+2.0mM | pFLMI |
| Peg3 | CTGGGTCAGCCATTCGATG | GCTCTGCAGCCTCCACTTC | TD55HSP+2.0mM | MspI |
| Peg10 | TGGTCAGTAGACGCATGAGC | CGCATGAAGCGCCTGGATCTTGGC | 59+2.0mM | HpyCH4III |
| Phf1 | GCTCCTGTGTTGTGTCTGTC | AGTCCATACGGCAGAGAGAG | TD65HSP | PstI |
| Piwil1 | GCATCGACTGTTACCATGAC | TCCACGGTCCTGAAAGAC | TD55HSP+0.5XQ | HpaI |
| Pkhd1l1 | CAATTCCATTTAGCAACTGT | CTGAAGGCTCTCGATTCT | TD65HSP+2.0mM | MnlI |
| Plagl1 | GTTCAACCGCAAGGACCAC | TCTTGGTGTGACGAGTGAGG | 54HSP+2.0mM | DdeI |
| Plekha5 | CCATCCCAGGATAATTGTAT | GGTCGCTCCTTCTTTTCTTC | TD55HSP | BfaI |
| Plp1 | GGTCCCAGAGAGAATTAGGCTAGA | GTACACAGGCGCAGCAGAGCA | 58HS | MseI |
| Pmfbp1 | AGGATGAAAAAGCTCAACAT | CAGCATCTTGAGGTCACTCT | TD55HSP | BsrI |
| Pofut2 | GGAGTCCACCTGAGAAGA | AAAAAAACCTGGCATGAG | TD55HSP+2.0mM | RsaI |
| Ppp1cb | GGAACAGATTCGGAGAA | AGCTCGACAAATCAAGTCTA | TD55HSP | PmlI |
| Ppp1r9b(#1) | GTCCGAGARAGAAGCAGAC | CGGAAGAAACTTCCTGTACTG | 62; 2.0mM | BclI |
| Ppp1r9b(#2) | GGA CCC CCT GGT CAAACG | GAC CCA ACC CTC TCCCGY GA | 62; 2.0mM | MseI |
| Prdm9 | CAAAGAACAAATGAGATCTGA | GTCTTYCTGTAATTGTTGAGATG | TD55HSP+0.5xQ | length |
| Prdx4 | TTYYGCGCTYGCKTGGTCATGGA | TCTTSGCTTTGCTTAGRTGCAG | 52HS | Fnu4HI |
| Prdx5 | GGATGATTCTTTGGTGTCTC | GGCAGACACAAATCAAGACT | TD65HS+0.5XQ | HpyCH4IV |
| Preb | ACCACTGCTACCTGAGTCTT | CTCTGTCGCCATCTACATAG | TD65HS+0.5XQ | DdeI |
| Prkcd | GGATGTGCAAAGAGAATATA | ATCCTGATGTTTCCTGTCAT | TD65HSP+2.0mM | DdeI |
| Prpf19 | GTCTCACCAAGGAAGTCACT | AGCCTGTGGTTTCAGAGTAG | TD65HS+0.5XQ | BsmAI |
| Prss37 | GAAATCTCAAAAGCAGAATC | CAGTCTTTGTCAGTCATCAC | TD55HS+1XQ | MboII |
| Ptpn13 | TTTTTCTTCTCTTTCCTCTG | AGTCCTCAAAGAGCAAATCT | TD65HSP+2.0mM | SmlI |
| Pygl | AAGAAAACCTTCGCCTATAC | ATCTTTGCTACCCCATTCAC | TD55HS+1XQ | NheI |
| GM9999 | GGC CTT CCA ATA ACCAC | CTGTGCAGTTGGGTGATCTT | TD65HSP | RsaI |
| Rbm5 | CTTCAACCTTTTTTTTTAAT | GTTGATGAAGAGGAAAACAG | TD55HSP+0.5XQ | AflII |
| Rnf20 | TTCATACTCTTTGCGGACCT | GAGGAAGTGGTTAAGGAGAC | TD65HS+0.5XQ | SacI |
| Rnf214 | AGCCTTCCTCAGATCCCTAC | AAAAGACAAAGAGCGAGGAT | TD55HSP | MboI |
| Rnf44 | ACATGATGCCACGAAGAC | GTTAGCCTTCAGCCACTT | TD65HSP+2.0mM | MseI |
| Scai | GCCTGAGTTGGTTGTTA | CAGTATTAAAGCGATGAGTA | TD55HSP | BsrI |
| Sec61a2 | TAAGACATGGATAGAAGTGT | AATTTTAGTTCTCTTGTGAA | 50HSP | Cell IEXTR |
| Sec62 | TAAAAGCCTGTTGCAGTAGT | GGTCATCGGGTTGATTAT | TD55HSP+2.0mM | MseI |
| Serinc1 | TGTTCTACCTTCTCCTCTCT | AAGAAAGCTCCAACAATAAT | TD65HSP+2.0mM | TaqI |
| Sh3gl3 | CGCGTTCACAGGCTTTAACT | TAGAGGCAGCATTGGACTAT | TD55HSP+O.5xQ | Cac8I |
| Siah2 | AATTTCTACATGCTGTCAGT | ATGTGTAAACAGGTCATCAC | TD55HSP+2.0mM | AvaI |
| Sik1 | GTTCCGCATCCCCTTCTT | CAAGGGCCTGCATGATAC | TD55HSP+0.5XQ | PstI |
| Sos1 | ATTCCAGAGCCTGAGCCAACAGA | CTCTTACTGTTCCAATAAATTCYTCCA | 62HS | Msp1 |
| Spag6 | AAGCAGAGATTTTCCCAGTT | CCTCCTGCGTTAACAATCAG | TD65+2.0mM | HphI |
| Spam1 | CCCAATTTTAAAGTCTCTTC | GCCTGTCACAATATTTTACA | TD55HSP | SfaNI |
| Sparc | CCCCAATGTTTAAAATGTTTGG | TTTCGTGTGTCTGTTACTTCCCT | TD55HSP+2.0mM | HhaI |
| Spata21 | CTCCAAGACCCGAGAGAC | GATCAGCACAGGATGGTATG | TD55HSP+2.0mM | MslI |
| Spata4 | TCTGTGAAATTGGGGTAGT | TTAAAAGTCAAGCTGGATAAC | TD55HSP+2.0mM | DdeI |
| Spsb3 | CAAGTGCTGCATAACAAG | ACCCACTGACATCATGTA | TD65HSP | BsmAI |
| Spz1 | TAGGTCGTCTGAGATGTCAC | AAGGGGNCAGTGATCCNATT | TD65HS+0.5XQ | Cell IEXTR |
| Sri | TGGAGACTTGTCGCCTTAT | TTCCACTCCTATCGCTGTC | TD55HSP | EarI |
| Srpk2 | GCATTCTGGGGAAGACTA | ATTGAAGAATTCCCGAGAA | TD55HS+2.5mM | Cell IEXTR |
| Sstr2 | CTCACCATCATCTGTCTTTGCTAC | TGGAGGTCTCCATTGAGGAG | TD55HSP | HhaI |
| Stk11 | GTGCTGTACAATGAGGAGAA | CTCAAGCAGAACTCCATCAC | TD55HS+1XQ | Cac8I |
| Tbc1d21 | CATGGGAACTTCACAGAGAC | CAGGGGATCTTTGTCATATA | TD55HSP | StyI |
| Tcp10b | AAAAATTCTACCCGGATGGC | GATGGTTTTGTCTCCGTTCC | 62hSP+0.2mM | 2%AG |
| Thbs1 | CTTTCACTTTGGGGCTTAGA | TCTCTATTCCAATGGCAATG | TD55HSP | NlaIII |
| Tmem111 | CTCCTGTCATCTGCTCCT | ATCCTGGTACTTCCTCAA | TD55HSP+0.5XQ | 2%AG |
| Tmem67 | CGCCTGTTCCATTATATC | GCTGTAAAACACCACCTTCT | TD55HSP | HpaI |
| Tpr | AGATCAAATGGAAAAAGAAA | TCCACTCCTTTTCTATCTCA | TD55HSP+2.0mM | RsaI |
| Tram1 | AATGGAGCGGTCACATC | TTCTGATTGCCAGTTGA | TD55HS+0.5XQ | BglII |
| Tyk2 | CAGAGGCAAGTGAATCTC | AGAGTCTTCCAGCGATTC | TD65HSP+0.5XQ | Fnu4HI |
| Ube2j1 | TAATGGACGATTTGAAGTAG | GCTCCCTCTCCTTTAGTT | TD55HSP+0.5XQ | 2%AG |
| Ube2k | TAAGAGATTTTGGCATTC | TCATCTTCTCATACGGTTTA | TD65HS | AleI |
| Ube3a | GCATAGTCCTCGGTCTG | ATCATACATCATTGGGTTAC | TD55HSP+2.0mM | BsaHI |
| Ubxn11 | GTGGAAAGGTGCTGAAGATC | GGAGATCATAGTGGAGACAC | TD65HS+0.5XQ | StyI |
| Ugp2 | TAACTCTGGCTTGCTCGACACCTT | TCCACCAGTCTCAGTTTGCCTTCA | 60HS+2.0mM | AvaII |
| Usp20 | TCTCGATGGGTTGGTTGT | CATGGTAGCCGCTGTG | TD55HSP | DdeI |
| Usp25 | CCTCTCCAGACTCAAACATC | TTCCTTATCTGTGCTTATCA | TD55HSP+4.0mM | 2%AG |
| Usp29 | TAGCTTAGTGCTTTGGTGTTGC | AGGGAGTTGACGTCAACGGAGT | 52HSP | HphI |
| Vars2 | GGATGCACATCATGAAGA | CTACAGCCCGCAGTATGT | TD65HSP | StyI |
| Xbp1 | TGGCCTTGTGATTGAGAACCAGGA | ACAGCGTCAGAATCCATGGGAAGA | TD65HSP | HindIII |
| Xist | CTCCCCCTTTAAGAGGGAACTTGA | TACCCGGAAGTGACCACAAT | 55 | BstNI |
| Xpnpep1 | TGGATTCGATGGCTCTG | ATGCTCTTCCGTGATGAT | TD55HSP | BfaI |
| Yme1l1 | ANATAACACAAGGAGCATTT | ATCAGAATTCGATGAGATGT | TD55HSP | MboII |
| Zdhhc3 | AACAACTGTGTCGGCNANAA | CAAGGAAATGAGAGCTATGT | TD55HSP | AvaI |
| Zfx | GCTCCGCAAGCTCTCAGGAAGT | AAGGAAAGGAGCATAAGTGATC | 52 | HaeII |
a
mM, concentration of magnesium chloride
b
CEL EXTR, Pimpkin M, Caretti E, Canutescu A, Yeung JB, Cohn H, Chen Y, Oleykowski C, Bellacosa, and Yeung AR. 2007. Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection. BMC Biotechnology 7, 29.
AG, agarose; AC polyacrylamide