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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mamm Genome. 2013 Dec 5;25(0):120–128. doi: 10.1007/s00335-013-9494-7

Figure 2. Ion Torrent amplicon sequencing workflow.

Figure 2

A schematic diagram of the steps involved in amplicon sequencing for genome scan analysis are shown. 150 to 200 bp around each SNP are amplified either individually or in a single multiplex PCR. Barcoded libraries generated from each mutant are then pooled to make a single library that is then amplified for sequencing on the Ion Torrent PGM.