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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Invest Dermatol. 2013 Nov 8;134(4):902–909. doi: 10.1038/jid.2013.474

Figure 3. Suppression of TPA-induced responses in PKD1-cKO mouse skin.

Figure 3

PKD1fl/fl or PKD1-cKO dorsal skin treated with a single dose of acetone (carrier control) or TPA (5 nmole/100 ul aceton) and 48 hrs later skin biopsies were taken and processed for histological analysis. Skin sections were stained with (a) H&E for histology, (c) antibody against proliferation marker Ki67 (peroxidase, dark brown staining) or (e) antibody against leukocyte marker S100A9 followed by Alexa-594 conjugated secondary antibody (red). Dapi (nuclear blue staining) was used as a counterstain. A representative of 6 mice from two separate experiments is shown. Scale Bars=100 μm. Graphs show the average thickness of epidermis (b), the number of Ki67-positive basal keratinocytes (d) and, the number of inflammatory S100A9-positive cells (f) in skin sections. Six different regions in sections prepared from three different mice were quantified. Values represent mean +SEM (n=3); * P< 0.001 TPA-treated PKD1-cKO versus TPA-treated control.