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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Chem Biol. 2014 Feb 16;10(4):251–258. doi: 10.1038/nchembio.1456

Figure 3. In vitro substrate profiling of NocTE.

Figure 3

(a) Schematic of NocTE reactivity towards various substrates. Top: Linear and activated penta- and tri- peptides are poor substrates for NocTE. Lower: Pre-formation of β-lactam at the seryl position results in NocTE epimerization and hydrolysis activity. (b) HPLC analysis of β-lactam-containing thioester substrates with excised NocTE. All of the β-lactam-containing substrates were processed by NocTE and only one product was formed, containing a C-terminal D-pHPG. Top traces show NocTE incubated with 8a and 8b, forming 1 and bottom traces show NocTE incubated with 9a and 9b, forming 14.