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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Chem Biol. 2014 Feb 16;10(4):251–258. doi: 10.1038/nchembio.1456

Figure 5. Kinetic analysis and determination of NocTE as a dual epimerase/hydrolase.

Figure 5

(a) Depiction of NocTE catalyzed conversion of epi-nocardicin G-SNAC (11a) and nocardicin G-SNAC (11b) to nocardicin G (1). (b) Plots of initial velocities vs. 11a and 11b concentration. Experiments were conducted in triplicate; data represents mean values ± standard deviation. (c) HPLC analysis of time points in competition experiments between 11b and 9b in the presence of NocTE, showing the appearance of 1 and 14.