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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Invest Dermatol. 2013 Oct 14;134(4):1119–1127. doi: 10.1038/jid.2013.420

Figure 3. Soluble P1 inhibited PDGF-BB binding to FN and FN-GFB-domains, and supported FN-null cell and AHDF survival.

Figure 3

a-d. 125I-radiolabeled PDGF-BB was incubated with FN or GST-tagged FN-GFB-domain-coated wells. After washing, radioactivity of bound PDGF-BB was determined by a γ-counter. e. Fluid-phase P1 supported FN-null cell survival on CBD/14. FN-null fibroblasts were plated at 4,000 cells/well(96-well-plates) pre-coated with 0.125μM GST-tagged CBD/14 in serum-free DMEM with FN-GFB peptide in solution±1nM PDGF-BB at 37°C for 3d. Viable cells in five-10xfields were counted in three wells (mean±SD, n=15). f. Fluid-phase P1 supported AHDF survival under serum-starvation conditions. AHDF were plated at 1,000 cells/well(96-well plates) in serum-free DMEM and cultured overnight, then peptide(10μM)±PDGF-BB(1nM) was added, cells cultured for 6d, and viable cells counted. Data shown are mean±SE (n=4).