TABLE 1.

Summary of the muropeptide composition of C. jejuni wild-type 81-176, Δpgp2, Δpgp2 complement (Δpgp2c), Δpgp1Δpgp2 double mutant, and Δpgp1 and the resultant Δpgp2 PG profiles of Pgp2 activity assays consisting of Δpgp2 PG incubated with and without Pgp2 enzyme preparations

Values represent the percentage area of each muropeptide from supplemental Table S2 calculated to give a total of 100%. Values indicated with an asterisk represent a greater than or equal to 20% difference in comparison with C. jejuni wild-type 81-176 or Δpgp2 PG to which no enzyme was added, and boldface, asterisked values indicate a greater than or equal to 30% change.

Muropeptide species	Percentage of peak area
	In C. jejuni strains					Following incubation with Δpgp2 PG
	81-176	Δpgp2	Δpgp2c	Δpgp1Δpgp2	Δpgp1a	Δpgp2 PG + buffer	Δpgp2 PG + Pgp2
	%	%	%	%	%	%	%
Monomers
Di	15.3	9.1*	14.8	10.2*	5.9	9.8	11.0
Tri	8.9	0.0*	7.4	0.0*	34.4	0.0	39.0*
Tetra	17.7	31.7*	19.6	30.7*	3.1	30.9	1.4*
Anhydro						0.0	6.2*
Total	41.6	40.8	41.8	40.9	43.4	40.7	51.4*
Dimers
TetraTri	16.0	0.0*	14.2	0.0*	37.0	0.0	24.5*
TetraTetra	31.1	46.8*	33.2	46.9*	14.7	49.1	16.1*
Tetra PentaGly5	0.7	0.8	0.7	1.0*	0.7	1.0	1.0
Anhydro	12.5	12.1	12.7	11.8	11.7	12.3	7.0*
Total	47.9	47.6	48.0	47.9	52.5	50.1	41.5
Trimers
TetraTetraTri	1.0	0.0*	0.8	0.0*	1.9	0.0	2.3*
TetraTetraTetra	9.5	11.6	9.4	11.2	2.3	9.2	4.8*
Total	10.5	11.6	10.2	11.2	4.2	9.2	7.1
Dipeptides (Total)	15.3	9.1*	14.8	10.2*	5.9	9.8	11.0
Tripeptides (Total)	17.3	0.0*	14.7	0.0*	53.6	0.0	52.0*
Tetrapeptides (Total)	67.0	90.5*	70.2	89.3*	40.2	89.7	36.5*
Pentapeptides (Total)	0.4	0.4	0.4	0.5	0.4	0.5	0.5
Anhydro chain ends (Total)	7.9	8.0	8.0	7.8	6.4	8.1	10.5*
Average chain length	12.7	12.5	12.5	12.8	15.7	12.4	9.5*
Degree of cross-linkage	31.0	31.6	30.8	31.5	29.0	31.2	25.5
Percentage of peptides in cross-links	58.4	59.2	58.2	59.2	56.6	59.3	48.6

^a Values for Δpgp1 are as published previously (11). For characterization of the differences between wild type and Δpgp1, see Frirdich et al. (11). Differences between the wild type and Δpgp1 are not highlighted with boldface and asterisked values.