FIGURE 3.
Effect of different Beclin 1 mutations on down-regulation of autophagy by Bcl-XL or M11. A, Western blots of MCF7 cell extracts indicating comparable expression levels of WT and mutant FLAG-tagged Beclin 1 constructs and of Bcl-XL and M11 in starvation and nutrient-rich conditions, with actin as a loading control. B–D, bar graphs representing light microscopy quantification of the number of discrete GFP-LC3 puncta per cell in GFP-positive MCF7 cells co-transfected with GFP-LC3, WT, or mutant Beclin 1 as indicated below the x axis and either no Bcl-2 homolog (B), Bcl-XL (C), or M11 (D).