TABLE 4.

3-Hydroxypropionate dehydrogenase activity of wild type, Δ/Δhpd1 mutant, and complemented strain (Δhpd1/HPD1)

Cultures were pregrown on YPD and shifted for 6 h to glucose and 8 h to acetate, valerate, or propionate medium. Activities are given in milliunits/mg, and mean values from three determinations with standard deviation are shown.

Strain	Glucose	Acetate	Propionate	Valerate
Wild type	1.2 ± 1.3	95.0 ± 9.7	230.1 ± 31.9	61.0 ± 8.9
Δ/Δhpd1	0 ± 0	0.2 ± 0.3	1.1 ± 1.0	0 ± 0
Δhpd1/HPD1	0.1 ± 0.2	36.9 ± 3.4	120.7 ± 6.3	52.3 ± 11.1