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PDX1.2 is modified in vitro at the N terminus. A, MALDI-TOF analysis of a tryptic digest of PDX1.2. The mass corresponding to the unmodified (m/z 2571.19 Da) and modified (m/z 2613.2 Da) N-terminal peptide alanine 2-lysine 26 is indicated. B, MALDI-TOF/TOF analysis of the modified and unmodified precursor ions m/z 2571.19 and m/z 2613.2, yielding a fragmentation pattern matching the predicted profile for the N-terminal peptide ADQAMTDQDQGAVTLYSGTAITDAK. Observed b ions are indicated.
