FIGURE 6.

Invalidation of 5-Htr6 activity rescues developmental retardations induced by exogenous 5-HT. Mouse embryos were explanted at E7.5 and subjected to in vitro culture for 72 h. 10 μm 5-HT, 5 μm methiothepin, and/or vehicle was added to the growth medium at E7.5. A, panels a–d, representative images of differently treated mouse embryos are shown. Arrows indicate brain regions with defective development. B, developmental progress of the embryos following different treatments was quantified by morphometric scoring. Significances were calculated using Student's t test; n = 4. ***, p < 0.001; **, p < 0.01. Error bars represent S.D. If no error bars are visible in the diagrams, no developmental variations between embryos of one experiment could be detected.