TABLE 2.
Dissociation constants of the isolated and tethered RRM domains
Shown are the dissociation constants of each construct by an EMSA and Trp lifetimes (FL) for RRM1, RRM2, RRM2c, and tRRMs to (UG)6, (TG)6, and T12. FL assays were only performed on RRM1 and tRRMs, whereas an EMSA was suitable for all constructs. Each experiment is an average of three independent experiments.
| RRM domain | Assay | Dissociation constant |
||
|---|---|---|---|---|
| (UG)6 | (TG)6 | T12 | ||
| nm | nm | nn | ||
| RRM1 | EMSA | 49.3 ± 12.0 | 232 ± 48 | >8000 |
| FL | 34.8 ± 14.1 | 630 ± 75 | >100,000 | |
| RRM2 | EMSA | 17,000 ± 3700 | >100,000 | >100,000 |
| RRM2c | EMSA | 2750 ± 480 | >100,000 | >100,000 |
| tRRMs | EMSA | 3.1 ± 1.3 | 20.2 ± 1.8 | >8000 |
| FL | 3.2 ± 2.2 | 279 ± 90 | >100,000 | |