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. 2014 Feb 12;289(12):8364–8374. doi: 10.1074/jbc.M113.523456

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — The expression of Tudor-SN and PPARγ are both enhanced in 3T3-L1 cells during adipogenesis. A, TCLs were harvested at different time points during the differentiation process as indicated and incubated with anti-Tudor-SN antibody, anti-PPARγ antibody, and anti-β-actin-antibody as a control. B, the band intensity was quantified using densitometry and normalized to the band intensity of β-actin. Shown are mRNA levels of Tudor-SN (C) and PPARγ (D) during adipogenesis. The expression levels were normalized to GAPDH levels.