FIGURE 6.

Tudor-SN affects H3 acetylation during adipogenesis. A, knock out of Tudor-SN reduces the acetylation level of histone H3. TCLs of MEF-WT and MEF-KO cells were immunoprecipitated (IP) with an anti-H3 antibody and then blotted with an anti-Ace-H3 antibody. B and C, TCLs of MEF-WT or MEF-KO cells at 0, 5, and 8 days of DMI treatment were immunoprecipitated with anti-PPARγ or anti-IgG as a negative control. The bound proteins were separated by SDS-PAGE and immunoblotted with anti-PPARγ, anti-PSF, anti-HDAC1, anti-HDAC3, and anti-Tudor-SN antibodies; 10% of the TCL was used as input.