FIGURE 11.

Scheme suggesting the traffic of HAS3 and the role of Rab10 in HA synthesis and cell adhesion. A, HAS3-containing transport vesicles have a constant flux between plasma membrane and cytoplasm with an average t_½ of ∼5–6 min. B, however, HAS3 with an ongoing HA synthesis remains stable in the membrane with a t_½ of 2–4 h. C, Rab10 enhances the early endocytosis of HAS3. When Rab10 is knocked down (red cross), the average time that HAS3 spends in the plasma membrane is increased, enhancing the probability of HA synthesis initiation. D, the HA coat enlarged by Rab10 knockdown counteracts the effect of cell surface adhesion molecules, resulting in impaired cell adhesion to collagen I.