FIGURE 7.

HAS3 follows clathrin-dependent endocytosis. A, EGFP-HAS3 showed a stronger colocalization with clathrin heavy chain (stained with anti-clathrin heavy chain antibody) in MCF7 cells, compared with mock transfection (EGFP). Scale bar, 25 μm. B, mRFP-HAS3 was also colocalized with fluorescein-transferrin, a marker for clathrin-dependent endocytosis, but not with Alexa Fluor® hydrazide 594 (Alx 594), a macropinocytosis marker. Scale bar, 10 μm. C, Pearson's correlation coefficient between clathrin heavy chain and EGFP-HAS3 (Rr = 0.46) was higher than EGFP only (Rr = 0.19). D, Pearson's correlation coefficient between mRFP-HAS3 and fluorescein-transferrin (Rr = 0.23) was significantly higher than EGFP-HAS3 and Alexa Fluor® hydrazide 594 (Rr = 0.07). Data in C and D represent mean ± S.E. (error bars) from three independent experiments with 10 images/group.