FIGURE 9.

Rab10 silencing decreases early endocytosis of HAS3. A, siRNA-treated MCF7 cells were transfected with EGFP-HAS3, fixed, and stained for EEA1, an early endosomal marker. In the Rab10 silencing group, there were fewer EGFP-HAS3-positive vesicular structures, except in the Golgi area, and relatively less colocalization of EGFP-HAS3 and EEA1, compared with the control and control siRNA groups. B, quantitative analysis showed that EGFP-HAS3 colocalization with EEA1 was significantly reduced in Rab10 silencing, compared with control groups. Data represent means ± S.E. (error bars) of three independent experiments with 15 images/group, taken with a 63× oil objective. *, p < 0.05; **, p < 0.005; ns, not significant (one-way ANOVA, Tukey's test). The colocalized panels are shown as 1-bit images. Scale bar, 10 μm.