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. 2014 Feb 5;289(12):8390–8401. doi: 10.1074/jbc.M113.534024

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Glis2 interacts with BBS1, BBS2, and BBS11/TRIM32. FLAG-tagged BBS proteins (BBS1–12) were transiently co-transfected with V5.Glis2 into HEK 293T cells. After 24 h of transfection, cells were lysed, and FLAG-tagged BBS proteins were immunoprecipitated with FLAG-M2 beads. The precipitates were loaded onto 10% SDS gel and analyzed by Western blotting using anti-V5 and anti-FLAG antibodies. V5.Glis2 interacted with F.BBS1, F.BBS2, and F. BBS11/TRIM32, but not with the other BBS proteins. Antibody heavy chains were marked with HC. IP, immunoprecipitation; WB, Western blotting.