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. 2014 Feb 5;289(12):8390–8401. doi: 10.1074/jbc.M113.534024

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Combined depletion of zGlis2 and zTRIM32 enhances cyst formation of the zebrafish pronephros. A, WT1-GFP transgenic zebrafish embryos, highlighting the proximal pronephros, were injected with control or zTRIM32-specific MO and scored for cyst formation at 55 h postfertilization. Knockdown of zTRIM32 resulted in pronephric cyst formation in a dose-dependent manner. The pronephric cysts of zTRIM32-deficient zebrafish embryos are marked with asterisks in the GFP-labeled pronephric ducts. No cyst formation was observed in zebrafish embryos injected with control MO. B, zebrafish embryos were injected with different MO combinations as indicated. Low doses of zTRIM32 MO enhanced the cyst formation in zebrafish embryos injected with zGlis2 MO.