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. 2014 Feb 5;289(12):8390–8401. doi: 10.1074/jbc.M113.534024

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — TRIM32 facilitates the accumulation of K63 ubiquitin chain-containing Glis2. Glis2 was co-expressed with HA-tagged, wild-type ubiquitin (Ubi-WT), HA-tagged ubiquitin K48R (Ubi-K48R), or HA-tagged ubiquitin K63R (Ubi-K63R) in HEK 293T cells. In A, cells were incubated for the last 2 h with 12 μm MG132. In B, cells were co-transfected with V5-tagged TRIM32 (V5.TRIM32). K63-linked ubiquitin chain accumulated in the presence of V5.TRIM32, but not after incubation with MG132, using a K63 specific antibody. IP, immunoprecipitation; WB, Western blotting.