FIGURE 5.

Manipulation of ST18 expression in INS cells. A, INS832/13 cells were transfected with control siRNA (siCtl) or two different ST18-specific siRNAs (siST18A and B), and, the following day, were exposed to palmitate (0.1 mm) and cytokines for an additional 24 h. ST18 expression was measured by qPCR. *, p < 0.05 versus corresponding control siRNA; **, p < 0.05 versus untreated cells. B, ST18 protein levels were determined by Western blot in cells treated as described above in the presence of palmitate. C, ST18 protein levels were determined by Western blot in cells transfected with either control (GFP) or pCMV6-ST18 (ST18) vectors for 24 h. Representative images of at least three experiments are shown.