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AMP/Fru-2,6-P2 synergism for wild-type and mutant FBPases. A–E, from assays using wild-type, His45, Arg46, Tyr186, and Cav− pFBPases, respectively. Reaction velocities as a function of AMP concentration are taken at 0 (○), 0.1 (□), 0.3 (▵), 1 (●), 3 (■), and 10 μm (▴) Fru-2,6-P2. F, from AMP inhibition assays with wild-type lFBPase at 0 (○) and 0.33 μm (+) Fru-2,6-P2. In all assays, the concentration of Fru-1,6-P2 is 20 μm, and that of Mg2+ is 10 mm.
