TABLE 2.
Fru-2,6-P2/AMP synergism of FBPases
Values in parentheses are uncertainties in the last significant digit.
| WT pFBPase | S45H pFBPase | T46R pFBPase | L186Y pFBPase | Cav− pFBPase | WT lFBPase | |
|---|---|---|---|---|---|---|
| Vmax (arbitrary units min−1) | 0.194 (4) | 0.88 (3) | 0.434 (8) | 0.495 (9) | 0.47 (1) | 0.48 (1) |
| (k′cat/(kcatKissa) (μm−2) | 0a | 0.0018 (4) | 0.0006 (1) | 0.0017 (2) | 0.0024 (3) | 0a |
| Kiba (μm) | 0.51 (4) | 0.30 (2) | 4.1 (5) | 0.28 (3) | 0.20 (2) | 0.21 (1) |
| (1/Kiaa + S/(KsKisaa)) (μm−2) | 0.78 (6) | 0.85 (6) | 1.7 (1) | 0.35 (3) | 0.72 (4) | 0.17 (2) |
| A0.5B=∞ (μm) | 0.42 (4) | 0.71 (6) | 1.4 (1) | 0.29 (3) | 0.60 (4) | 0.14 (2) |
| A0.5B=0 (μm) | 3.9 (3) | 2.4 (2) | 11 (1) | 2.3 (2) | 2.0 (2) | 2.1 (2) |
| A0.5B=0/A0.5B=∞ | 9 (1) | 3.4 (9) | 8 (1) | 8 (2) | 3.3 (6) | 15 (4) |
a Value fixed to zero, reflecting full inhibition at saturating concentrations of AMP.