FIGURE 3.
Claudin and E-cadherin expression is transcriptionally regulated by integrin β1 and is independent of confluency and anchorage. A, cell lysates from β1fl/fl and β1−/− PTCs either sparsely grown or grown to confluency were immunoblotted for the proteins shown in the figure. B, β1fl/fl and β1−/− PTCs were grown in the presence or absence of polyhema (PH), and the amount of E-cadherin and claudin-2 and -7 was analyzed by Western blotting. The lanes are separated by lines to demarcate samples run on the same gel but not in the same order as shown in the figure. C, quantitative RT-PCR analyses for claudin-2, claudin-7, and E-cadherin was performed on cDNA synthesized from RNA isolated from β1fl/fl and β1−/− PTCs. The results were normalized to expression by β1fl/fl PTCs. Mean measurements of three independent experiments are shown; *, p ≤ 0.05 between β1fl/fl and β1−/− PTCs.