FIGURE 8.
Kidneys of γgt-cre:β1flox/flox mice have morphologically normal kidneys but have an isosmolar diuresis and an inability to concentrate urine after water loading and subsequent deprivation. A and B, histology of kidney cortex of 6 week β1flox/flox (A) and γgt-cre:β1flox/flox (B) mice is normal (200×). C, 24 h water intake (*, p < 0.01) and (D) 24 h urine output is increased in γgt-cre:β1flox/flox mice (*, p < 0.01). E, baseline urine osmolality is similar in β1flox/flox and γgt-cre:β1flox/flox mice. F, mice (6 in each group) were water loaded with an intraperitoneal injection of 2ml of water 18 h prior to and at the commencement of the experiment. At 4 h, γgt-cre:β1flox/flox and β1flox/flox mice diluted their urines appropriately; however, γgt-cre:β1flox/flox mice were unable to concentrate their urines 6 and 8 h after water deprivation (*, p < 0.01).