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. 2014 Jan 30;289(12):8620–8632. doi: 10.1074/jbc.M113.543033

FIGURE 1.

FIGURE 1.

QND pH titration. Main panel, QND (20 μm) spectral changes during stepwise addition of NaOH in 1 mm potassium phosphate buffer at 25 °C. Spectra at pH 7.6 (thick line) and pH 11.5 (dashed line). The inset show the data fitting at 335 and 380 nm to Equations 1 and 2, respectively.