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pH dependence of steady-state kinetic constants for QND oxygenation by WT HOD and its D126A and H251A variants. pH dependence of kcat (A) and kcat/Km (B) for WT (●), D126A (□), and H251A (▴). All reactions were performed in air-saturated 0.1 m MTE buffer, 5 mm NaCl and 2 mm EDTA at 20 °C. The errors considered in the measured parameters were taken larger than the standard deviation between four replicates and the numerical errors after fitting.
