TABLE 1.

Steady- and transient-state kinetic constants for WT HOD and its variants at different pH values

^a Steady-state constants were determined in 0.1 m MTE Buffer containing 2 mm EDTA, 5 mm NaCl at 20 °C.

^b Transient-state constants were determined in the former buffer at 5 °C.

^c nd means not determined because of no QND dependence; values are shown at 1 mm QND. Means ± S.D. from curve fitting are provided.