FIGURE 1.

Glrx overexpression attenuates EC migration and network formation, as well as aortic sprouting. A, Western blot analysis of human Glrx transgene or endogenous Glrx in WT and TG mouse microvascular EC (mEC). B and C, representative (B) and quantitative (C) blinded scoring of EC network formation in WT and TG mEC seeded onto Matrigel in the presence and absence of VEGF (50 ng/ml) (n = 6; *, p < 0.05). D and E, representative (D) and percentage (E) wound closure of WT and TG mEC after 18h VEGF (50 ng/ml) treatment, measured at four predetermined locations (n = 3; *, p < 0.05). F and G, representative (F) and quantitative (G) assessment of sprouting of EC from isolated WT and TG aortae after 6 days cultured on Matrigel in DMEM 2% FBS with and without VEGF (50 ng/ml) (n = 4; *, p < 0.05).