FIGURE 6.

Glrx decreases p65-glutathione adducts in ischemic muscle and endothelial cells. A–D, biotin switch assay showing the biotin labeled S-glutathionylated cysteines within p65 (pull down) in WT (A) and Glrx TG non-ischemic (NI) and ischemic (ISC) muscle 4 days after HLI, and in hEC overexpressing Glrx or LacZ (C) under normoxic or hypoxic (0.1% 24 h) conditions with respective quantification of GSH adducts (B and D) normalized to p65 input, samples prior to incubation with streptavidin beads (n = 3–4; *, p < 0.05; **, p < 0.01). IB, immunoblot.