FIGURE 5.

Inhibition of IL-6 inducing activity of hexaacylated LPS from lgtB⁻ mutant in MM6 cells by the pentaacylated LPS from lgtB⁻pagL⁺ and galE⁻lpxL1⁻ mutants. Asterisks indicate statistically significant differences (p < 0.05) in the level of IL-6 expression between MM6 cells stimulated with lgtB⁻ LPS only and those stimulated with a mixture of lgtB⁻ LPS and lgtB⁻ pagL⁺ LPS (A), or between lgtB⁻ LPS only and those stimulated with a mixture of lgtB⁻ LPS and galE⁻ lpxL1⁻ LPS (B) as determined by the Mann-Whitney test by using GraphPad Prism 6.0 statistical software (GraphPad Software, Inc.). Bars represent the average of quadruplicate determinations and error bars the standard deviation.