FIGURE 8.

Secretion of IP-10 (A) and IL-10 (B) by MM6 cells after stimulation with mutant LPS of N. meningitidis. MM6 cells were stimulated with serial dilutions of LPS preparations from the lgtB⁻ (open circles), lgtB⁻ pagL⁺ (closed squares), or galE⁻ lpxL1⁻ (closed diamonds) mutants or of LPS from the lgtB⁻ pagL⁺ mutant that had been previously incubated in neutral 5% TEA at RT for 3 h (closed triangles) or subjected to mild alkaline hydrolysis in 5% TEA at 45 °C for 3 h (open inverted triangles). Control cells were incubated in supplemented IMDM only (asterisks). Cell incubations were performed for 16–18 h at 37 °C in a 5% CO₂-humidified atmosphere. IP-10 and IL-10 concentrations in supernatants were determined by ELISA. Each data point represents the average of triplicate determinations; error bars indicate the standard deviation.