. Author manuscript; available in PMC: 2014 Mar 21.

Published in final edited form as: J Fish Dis. 2013 Jan 24;36(8):721–733. doi: 10.1111/jfd.12073

Table 2.

Cyprinus carpio proteins identified by ESI-MS analysis

Accession no.	Protein description	Score	Mass [Da]	Matches	Coverage [%]
(a) Fifty-four C. carpio proteins identified by electrospray analysis from gel slices originating in symptomatic fish samples grouped into 7 aforementioned categories
Michel et al. 2010 or homolog proteins
gi\|28628941	Elongation factor 1-alpha	128	50 325	3	6.1
gi\|l7369826	Lactate dehydrogenase A chain	115	36 539	2	5.4
gi\|l5628189	Heat shock protein 90 alpha	28	25 336	1	5.3
gi\|l4388583	Warm-temperature acclimation-related 65-kDa protein	78	50 640	3	6.6
gi\|218511593	RAB8A	23	23 823	1	5.3
Cytoskeletal proteins
gi\|42560193	Beta-actin	496	42 068	24	25.1
gi\|1703140	Alpha-actin-1	326	42 274	14	18.8
gi\|13365501	Integrin beta-2 chain	173	87 597	5	7.3
gi\|6226787	Vimentin	56	52 516	4	3.5
Host defence proteins
gi\|4126587	Complement C3-H1	223	184 985	6	4
gi\|9453863	Complement C4-1	67	193 004	1	0.8
gi\|9453865	Complement C4-2	98	194 643	3	1.9
gi\|3399699	Natural killer cell enhancing factor	44	22 410	1	5.5
gi\|297718575	Granzyme A/K	26	29 149	2	4.3
gi\|7939556	Lysozyme C	49	17 241	2	11.7
gi\|2829695	Granulin-3	47	6995	2	24.6
Protein modification enzymes or inhibitors
gi\|439153	Serine protease inhibitor	288	45 960	12	13.4
gi\|416561	Alpha-1-antitrypsin homolog	372	42 044	22	23.7
gi\|56709493	Putative glyceraldehyde-3-phosphate dehydrogenase	21	33 540	2	2.2
gi\|4027925	Creatine kinase M1-CK	37	42 980	1	3.4
gi\|73762632	Carbonic anhydrase	80	28 716	3	11.9
gi\|373503076	Glutamate dehydrogenase	910	54 750	46	47.1
gi\|112901127	Glutathione S-transferase rho	35	26 700	1	6.6
gi\|152925970	Ubiquitin fusion protein	279	14 987	19	41.4
gi\|91798526	Proteasome activator PA28 subunit	37	28 579	1	2.8
gi\|217272706	Monoamine oxidase	442	59 621	13	16.3
gi\|6690508	Janus kinase 3	22	131 078	1	0.7
gi\|300676317	Catalase	21	49 187	1	3.1
Globins
gi\|2208883	Alpha-globin	529	15 421	35	52.4
gi\|2208891	Alpha-globin	488	15 566	35	54.5
gi\|2208889	Alpha-globin	390	15 574	28	61.5
gi\|122392	Alpha-globin	595	15 437	45	62.2
gi\|6009727	Alpha-2-macroglobulin-1	631	162 274	22	11.8
gi\|6009729	Alpha-2-macroglobulin-2	541	157 876	18	10
gi\|6009731	Alpha-2-macroglobulin-3	434	88 297	14	15.1
gi\|140368180	Alpha-2-macroglobulin 4	368	101 340	12	11.4
gi\|22135548	Beta-globin	584	16 520	41	63.3
gi\|2208895	Beta-globin	732	16 617	67	87.2
gi\|1644253	Beta-globin	718	16 645	63	87.2
gi\|1644251	Beta-globin	706	16 542	68	87.2
gi\|3953518	Immunoglobulin heavy chain	1053	50 331	54	47.6
gi\|55700034	Immunoglobulin heavy chain	78	40 300	4	6.4
gi\|85067845	Myoglobin isoform 2	29	16 278	1	5.4
Transferrins
gi\|18034630	Transferrin variant A	1882	75 595	100	47.1
gi\|189473163	Transferrin variant F	986	75 036	45	26
gi\|189473165	Transferrin variant G	1003	75 083	47	26.9
Unclassified
gi\|29501366	Fetuin short form	43	34 332	2	7
gi\|13445027	Apolipoprotein A-I	83	20 797	4	16.1
gi\|435737	Glial fibrillary acidic protein	119	24 944	69	9.3
gi\|151558991	Vitellogenin B1	2221	148 683	100	39.6
gi\|52782167	Vitellogenin B2	964	179 640	48	13.4
gi\|14009437	Mitochondrial ATP synthase	32	59 699	1	2.2
gi\|47605558	Mitochondrial synthase subunit beta	71	55 327	2	4.1
gi\|10566900	Myeloid protein-1	35	17 849	1	7.5
(b) Fifty-two C. carpio proteins identified by electrospray analysis from unmodified lysate solution originating in symptomatic fish samples grouped into 7 aforementioned categories
Michel et al. 2010 or homologs
gi\|28628941	Elongation factor 1-alpha	97	50 325	5	6.7
gi\|l7369826	Lactate dehydrogenase A chain	110	36 539	2	6.3
gi\|55669145	Lactate dehydrogenase B-type subunit	150	36 669	3	9
gi\|33598990	Constitutive heat shock protein HSC70-2	329	70 779	12	12.3
gi\|218511593	RAB8A	26	23 823	1	5.3
Cytoskeletal proteins
gi\|42560193	Beta-actin	457	42 068	15	24.8
gi\|6226787	Vimentin	127	52 516	6	3.5
Host defence proteins
gi\|4126587	Complement C3-H1	362	184 985	9	5.2
gi\|4126593	Complement C3-S	179	185 561	5	2.9
gi\|3399699	Natural killer cell enhancing factor	93	22 410	3	9.5
gi\|84569882	Natural killer cell enhancing factor	251	22 014	10	47.7
gi\|165880803	MIF	26	12 525	1	14.8
gi\|47117021	Lysozyme g	88	20 425	3	10.8
Protein modification enzymes or inhibitors
gi\|439153	Serine protease inhibitor	83	45 960	2	9
gi\|416561	Alpha-1-antitrypsin homolog	261	42 044	7	15.6
gi\|56709493	Putative glyceraldehyde-3-phosphate dehydrogenase	146	33 540	2	9.2
gi\|4027925	Creatine kinase M1-CK	34	42 980	1	1.6
gi\|73762632	Carbonic anhydrase	285	28 716	7	40.4
gi\|373503074	Glutamate dehydrogenase	63	54 737	2	3.1
gi\|112901127	Glutathione S-transferase rho	51	26 700	1	4.4
gi\|95832156	Pi-class glutathione S-transferase	69	23 794	2	10.1
gi\|300676299	Glutathione peroxidase 1	37	19 182	1	10
gi\|343481094	Glutathione synthetase	22	41 342	1	2.5
gi\|353351682	Trypsin 1	114	26 940	1	6.6
gi\|117518748	Enolase	21	16 206	1	4.1
Globins
gi\|2208891	Alpha-globin	527	15 566	44	73.4
gi\|2208883	Alpha-globin	494	15 421	35	69.2
gi\|2208887	Alpha-globin	492	15 449	36	69.2
gi\|2208889	Alpha-globin	372	15 574	24	69.2
gi\|122392	Alpha-globin	659	15 437	56	81.1
gi\|1644251	Beta-globin	652	16 542	47	78.4
gi\|2208895	Beta-globin	568	16 617	46	70.9
gi\|22135548	Beta-globin	485	16 520	26	54.4
gi\|3953518	Immunoglobulin heavy chain	177	50 331	5	12.8
gi\|85067845	Myoglobin isoform 2	86	16 278	2	12.9
Transferrins
gi\|18034630	Transferrin variant A	420	75 595	17	12.3
gi\|189473165	Transferrin variant G	321	75 083	12	10.2
Unclassified
gi\|29501366	Fetuin short form	64	34 332	3	7.3
gi\|13445027	Apolipoprotein A-I	226	20 797	7	32.2
gi\|435737	Glial fibrillary acidic protein	417	24 944	41	28
gi\|435739	Glial fibrillary acidic protein	504	24 531	44	33.2
gi\|49356890	Glia-derived neurotrophic factor	25	11 004	1	8.6
gi\|2252655	Nephrosin precursor	340	31 629	13	27.5
gi\|281333450	Heart-type fatty-acid-binding protein	156	14 704	5	21.8
gi\|15778562	Vitellogenin	294	148 794	12	7.8
gi\|84569880	Translationally controlled tumour protein	84	19 152	2	8.2
gi\|281429776	Adipocyte fatty-acid-binding protein	124	15 278	2	16.4
gi\|1173446	Somatoliberin	28	4976	1	17.8
gi\|585105	Ependymin	385	24 433	67	41.9
gi\|40549335	Cytochrome P4501C1	24	59 583	1	1.3
gi\|219935425	Mineralocorticoid receptor	24	107 380	1	0.5
gi\|169674670	Liver-basic fatty-acid-binding protein b	24	14 255	1	15.9
(c) Twelve C. carpio proteins identified by electrospray analysis from unmodified lysate solution originating in asymptomatic fish samples grouped into 7 aforementioned categories
Michel et al. 2010 or homolog proteins
gi\|32394421	Muscle-specific heat shock protein Hsc70-1	51	70 660	2	6.1
Cytoskeletal proteins
gi\|42560193	Beta-actin	78	42 068	4	8.8
gi\|1703140	Alpha-actin-1	77	42 274	3	9
gi\|6226787	Vimentin	32	52 516	1	1.5
Host defence proteins
gi\|7939556	Lysozyme C	264	17 241	20	43.4
gi\|313509543	Toll-like receptor 22	30	109 136	3	0.7
gi\|2829694	Granulin-2	43	7215	1	17.5
Protein modification enzymes or inhibitors
gi\|152925970	Ubiquitin fusion protein	99	14 987	5	24.2
Globins
gi\|122392	Alpha-globin	88	15 437	4	16.1
gi\|85067845	Myoglobin isoform 2	25	16 278	1	5.4
Unclassified
gi\|435737	Glial fibrillary acidic protein	80	24 944	30	5.1
gi\|162423638	Cyclin B	27	45 068	1	2

MIF, migration inhibitory factor.

The 54 and 52 identified proteins from either gel slices or unmodified samples, respectively, were classified into seven groups that include: Michel et al. 2010, cytoskeletal, host defence, protein modification or inhibitors, globins, transferrins, unclassified. Proteins that showed up in both analyses are in bold.