. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Mol Microbiol. 2013 Nov 21;90(6):1324–1338. doi: 10.1111/mmi.12437

Table 4.

Oligonucleotides used

Name	Sequence, 5'→3'	Purpose
plAm-Hyg-up	GTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCCCGTAGAGATTGGCGATCCC	Replacement of aac(3)IV in
p2Am-Hyg-rp	TCATGAGCTCAGCCAATCGACTGGCGAGCGGCATCGCATCAGGCGCCGGGGGCGGTGTC	pOOB47a with hyg
aac-up	GGGCCACAGGCAGAGCAGA	Diagnostic primers to amplify
aac-rev	GAGCCACCTGTCCGCCAAG	Diagnostic primers to amplify
apra_i	AGTTGTCTCTGACACATTCT	aac(3)IV and moeB4 flank
moeB4up	CGCGCGATCATGTAAGTCTGCAC	aac(3)IV and moeB4 flank
moeB4-red-up	GAAGGCAAGGCCCCGGTTGTGGCGGTGCTGACCGTCACCATTCCGGGGATCCGTCGACC	Replacement of moeB4 within
moeB4-red-rp	GATCGCGGCGGTCAGCTCCGGTTCGCCCCAGTAGCCGAGTGTAGGCTGGAGCTGCTTC	moeno5 with aac(3)IV-oriT
moeB4HY-red-up	GAAGGCAAGGCCCCGGTTGTGGCGGTGCTGACCGTCACCCCCGTAGAGATTGGCGATCCC	Construction of pOOB64bd
moeB4HY-red-rp	GATCGCGGCGGTCAGCTCCGGTTCGCCCCAGTAGCCGAGCCAGGCGCCGGGGGCGGTGTC
moeH5-up-aac	AGGCCGCCCTCCAGCCCCTGCTGGACGCCCGATGACGGTATTCCGGGGATCCGTCGACC	Replacement of moeH5 within
moeH5-rp-aac	TCTCGTGAAGTGGGGGTCTGCGGCGGTCCGGCCCCGCTATGTAGGCTGGAGCTGCTTC	moeno38 with aac(3)IV-oriT
moeGT1XbaIup-int	AAATCTAGAACCAGCGGTACATGGCCCTCATG	Cloning of moeH5
h5exp6hisEcoRI	AAAGAATTCAGTGGTGGTGGTGGTGGTGCGGCCGGCGGCGGGCCCTTCG	+ 6 His tag
moeGTlXbalup con73start	AAATCTAGATGCTCCTGGACACCCTCTCCAAAAAGCTTGCTGATGTCTCGTGAAGTGG	Cloning of moeH5