Figure 4. There is increased peripheral immunosurveillance of CNS neoepitopes under neuroinflammatory conditions.

Nine days post-EAE induction, mice received 5 × 10⁵ neoepitope-specific T cells. FACS was performed within 1–4 days of disease onset. (A) Histograms show percentage of CFSE^low/int/CFSE^high cells. (B) Column graphs indicate mean # of CFSE^low/int LFA-1^high CD8⁺/CD4⁺ Thy1.1⁺ cells/gram tissue. Error bars, SEM. *, p < 0.05; **, p < 0.01. N = 7 littermates, n = 5 PLP-OP and n = 11 CNP-OP mice (3 experiments).