FIG. 1.

Characterization of injury severity caused by CCI. Mild CCI does not cause a significant loss of neurons in the CA1 region 24 hours or 7 days after injury. (A) Photomicrograph of Nissl-stained brain coronal slice. Indicated are the sites of impact and the ipsilateral (Ipsi) and contralateral (Contra) CA1. (B) Representative photomicrographs from Nissl-stained sections showing BLA cells from the ipsilateral (top) and contralateral (bottom) sides of sham, 1-day CCI, and 7-day CCI animals, respectively. Total magnification is 630×; scale bar, 50 μm. (C) Group data (mean±standard error; n=8 for each group) of stereological estimation of the total number of Nissl-stained neurons in the CA1. There were no significant differences in neuronal number between sham and 1-day or 7-day CCI, on either ipsilateral or contralateral sides, using Student's t-test. CCI, controlled cortical impact.